Recently, the most important issues in the tissue engineering field are to mimic the natural extracellular matrix (ECM) and to manufacture the customized structures with patient-specific morphology. These are the critical factors for regulating functions of cultured cells and being used as the clinical-level applications. In these respects, nanoscale fabrication and 3D printing technologies have attracted much attention from the scientists and engineers in the relevant fields. In this presentation, we are going to introduce new hybrid bio-printed constructs, which consist of 3D printed parts and electrospun nanofiber mats. When it comes to electrospinning process, it could formulate not only randomly-network fibrous mat of it natural processed form but also highly-oriented fiber arrays. Depending on the fiber orientation, the cultured cells on the scaffolds could be modulated in terms of their biological functions and behaviors. The biomaterials for 3D printing could be classified to two groups; i) thermoplastic rigid polymers and ii) hydrogel-based bio-ink. When the thermoplastic biomaterials, for which polycaprolactone (PCL) was used in our studies, were combined with the electrospun mats, they were effectively supportive to sustain the morphologies of nanofiber mats. Since the supportive constructs were fabricated in the way of CAD/CAM-fashioned process, they were expected to overcome various obstacles in practical uses of the fragile nanofiber scaffold and meet the needs for clinical applications in specific surgeries. As for the hydrogel-based materials, which physically involved biologically-living cells, they were 3D-printed in the layer-by-layer manner. When the layering of bio-inks were processed with insertion of nanofiber mats between the hydrogel layers, the integrated constructs had enhanced properties in terms of structural resolution as well as mechanical toughness and stiffness. The improved performances of fiber-reinforced hydrogel constructs were expected to achieve the morphological and biophysical mimicry of native soft tissues. Taken together, the combinatory fabrication techniques involving 3D printing and electrospinning would allow for a wide range of feasible applications in the scaffold-based tissue engineering.